After having your bicycle allocated and being introduced to your group, your guide will start by leading you through the Circus Maximus. This is a great opportunity to test your bike and make sure you are comfortable for the rest of the tour. The first leg of the tour is along the Appian Way, an ancient Roman road running through the countryside past Roman tombs and villas. Peddle to the old city walls and the gate of St. Sebastian. It is said that St. Peter was to have passed through here on his way out of Rome. Following a short stretch along cobbled paving stones and past the obscured ruins of an old Roman villa, reach the first real breath of fresh air in the sanctuary of the Catacombs of St. Callisto.

Admire the Catacombs of St. Callisto from the outside, and listen as your guide describes the importance of this ancient Christian site. Cycling a bit further, arrive at the Tomb of Cecilia Metella and the first stretch of original stone paving used to build the Appian Way.

Continue over this 2,300-year-old road to get the most out of your Appian Way Bike Tour experience. There are trails and paths running alongside this bumpy road allowing the group to bike easily for most of the way until reaching the Park of the Aqueducts. At the Park of the Aqueducts, get up close to the ruins of Rome's most impressive aqueduct, the Aqueduct Claudius. It towers overhead and offers an amazing backdrop throughout this second leg of the tour as you make your way to Cafarella Valley. Visit a natural spring for a scenic break in Cafarella Valley. The area is home to many Roman ruins such as Ninfeo Egeria. At the natural spring, take time to use the toilet and try some of the mineral water. You'll also have the chance to sample local cheese and wines while there.